Mongolia is the Przewalski's horse's native habitat; the animals were declared extinct there in the 1970s. In the past, Port Lympne has returned the Przewalski's horses to the wild in both Mongolia and China. Owned by the Aspinall Foundation, the profits from the hotel and reserve go toward the foundation's commitment to conservation, through captive breeding, education and reintroduction. The Foundation is working in some of the world's most fragile environments to save endangered animals and return them to the wild.

In 2008, the International Union for Conservation of Nature changed the Przewalski's horse's classification to critically endangered in 2008; their classification changes again in 2011 to endangered after horses were reintroduced into the wild.